Graphene Enabled Low?Noise Surface Chemistry for Multiplexed Sepsis Biomarker Detection in Whole Blood

Affinity-based electrochemical (EC) sensors offer a potentially valuable approach for point-of-care (POC) diagnostics applications, and the detection of diseases, such as sepsis, that require simultaneous multiple biomarkers, but their development has been hampered due to biological fouling EC noise. Here, an sensor platform enables sepsis biomarkers simultaneously by incorporating nanocomposite coating composed crosslinked bovine serum albumin containing network reduced graphene oxide nanoparticles prevents biofouling while maintaining electroconductivity is described. Using coated planar gold electrodes, sensitive procalcitonin (PCT) constructed validated in undiluted serum, which produced excellent correlation with conventional ELISA (adjusted r2 = 0.95) using clinical samples. A single multiplexed three different biomarkers—PCT, C-reactive protein, pathogen-associated molecular patterns—is also developed, exhibits specific responses within clinically significant range without any cross-reactivity. This analytes human whole blood, it can be applied detect target analyte appropriate antibody pair. Thus, this nanocomposite-enabled may tool wide POC diagnostics.